The group of Yunhong SONG at CAS Institute of Industrial Biotechnology in Tianjin cloned a soluble enzyme I (SHI) from Pyrococcus furiosus and overexpressed it in Thermococcus kodakarensis. Purification was achieved in one step using a nickel-loaded column. The rSHI purified by this method was coupled with an FMN-containing diaphorase (DI) to form a new electron transport chain. which utilized hydrogen as a reducing agent to regenerate NADH, used to convert pyruvic acid into lactic acid.
CAS news release, November 5, 2018